Assessment of Cytomorphological Differences in Sorghum Fertility Restoration

Sorghum (Sorghum bicolor L. Moench) is ranked fifth as a cereal crop after maize, rice, wheat, and barley based on global cultivation area. However, heat drought stresses cause improper fertility restoration inefficient pollination, severely affecting sorghum productivity. The discovery of cytoplasmic male sterility (CMS) milestone for commercializing hybrids. This study assessed the pollen in vitro germination percentage female lines F1 hybrids using two years pooled data with multivariate analysis. principal component analysis (PCA) showed that PC1 represented 82.8% variation, whereas PCA revealed significant genetic divergence 97.1%. Agglomerative hierarchical clustering marked cluster II genotypes have high contribution, which can generate superior high-yielding Three male-sterile exhibited 100% sterility, morphological attributes, viz., pinpointed, flattened, low anther extrusion, starch-digested pollens. Pollen behavior nine were fully fertile, eighteen partially three completely sterile amongst thirty findings this will facilitate identification potential restorers exploitation breeding programs.